EU-kalat

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EU-kalat is a study, where concentrations of PCDD/Fs, PCBs, PBDEs and heavy metals have been measured from fish

Question

The scope of EU-kalat study was to measure concentrations of persistent organic pollutants (POPs) including dioxin (PCDD/F), PCB and BDE in fish from Baltic sea and Finnish inland lakes and rivers. .

Answer

The original sample results can be acquired from Opasnet base. The study showed that levels of PCDD/Fs and PCBs depends especially on the fish species. Highest levels were on salmon and large sized herring. Levels of PCDD/Fs exceeded maximum level of 4 pg TEQ/g fw multiple times. Levels of PCDD/Fs were correlated positively with age of the fish.

Mean congener concentrations as WHO2005-TEQ in Baltic herring can be printed out with the Run code below.

+ Show code- Hide code # This code is Op_en on page [[EU-kalat]]. library(OpasnetUtils) library(ggplot2) eu <- Ovariable("eu", ddata = "Op_en3104", subset = "POPs") eu <- EvalOutput(eu) #levels(eu$POP) eu <- eu[eu$Fish_species == "Baltic herring" , ] tef <- Ovariable("tef", ddata = "Op_en4017", subset = "TEF values") tef <- EvalOutput(tef) colnames(eu@output)[colnames(eu@output) == "POP"] <- "Congener" levels(eu$Congener) <- gsub("HCDD", "HxCDD", levels(eu$Congener)) levels(eu$Congener) <- gsub("HCDF", "HxCDF", levels(eu$Congener)) levels(eu$Congener) <- gsub("CoPCB", "PCB", levels(eu$Congener)) #levels(eu$Congener) euteq <- eu * tef #head(euteq@output) temp <- aggregate(euteq@output["Result"], by = c(euteq@output["Congener"], euteq@output["Catch_location"]), FUN = mean) temp2 <- temp temp2$Result <- temp2$Result / sum(temp2$Result) cat("Congener TEQ profile of Baltic herring.\n") oprint(temp2) ggplot(temp, aes(x = Congener, y = Result, fill = Congener)) + geom_bar(stat = "identity") + facet_wrap(~ Catch_location) + labs(x = "Congener", y = "TEQ concentration (pg/g fat)") + theme_gray(base_size = 18) + coord_flip()

Rationale

Data

Data was collected between 2009-2010. The study contains years, tissue type, fish species, and fat content for each concentration measurement. Number of observations is 285.

There is a new study EU-kalat 3, which will produce results in 2016.

Calculations

Preprocess

• Preprocess model 22.2.2017 [4]
  • Objects used in Benefit-risk assessment of Baltic herring and salmon intake
• Model run 25.1.2017 [5]
• Model run 22.5.2017 with new ovariables euRaw, euAll, euMain, and euRatio [6]
• Model run 23.5.2017 with adjusted ovariables euRaw, eu, euRatio [7]
• Model run 11.10.2017: Small herring and Large herring added as new species [8]

+ Show code- Hide code # This is code Op_en3104/preprocess on page [[EU-kalat]] library(OpasnetUtils) library(ggplot2) library(reshape2) # Divide herring into small and large (as new "species") euRaw <- Ovariable("euRaw", ddata = "Op_en3104", subset = "POPs") euRaw <- EvalOutput(euRaw) temp <- euRaw[euRaw$Fish_species == "Baltic herring" , ] temp$Fish_species <- as.factor( ifelse( as.numeric(as.character(temp$Length_mean_mm)) >= 170, "Large herring", "Small herring" ) ) euRaw <- combine(euRaw, temp, name = "euRaw") # levels(TEF$Group) #[1] "Chlorinated dibenzo-p-dioxins" "Chlorinated dibenzofurans" #[3] "Mono-ortho–substituted PCBs" "Non-ortho–substituted PCBs" eu <- Ovariable( "eu", dependencies = data.frame( Name=c("euRaw", "TEF"), Ident=c(NA,"Op_en4017/initiate") ), formula = function(...) { eu <- euRaw[,c(1:4, 18, 19)] # THL code, Matrix, Congener, Fish species colnames(eu@output)[1:4] <- c("THLcode", "Matrix", "Compound", "Fish") temp <- oapply(eu * TEF, cols = "Compound", FUN = "sum") colnames(temp@output)[colnames(temp@output)=="Group"] <- "Compound" eu <- combine(eu, temp) eu$Compound <- factor( # Compound levels are ordered based on the data table on [[TEF]] eu$Compound, levels = unique(c(levels(TEF$Compound), levels(eu$Compound))) ) eu$Compound <- eu$Compound[,drop=TRUE] return(eu) } ) euRatio <- Ovariable( "euRatio", dependencies = data.frame(Name=c("eu")), formula = function(...) { euRatio <- eu[ eu$Compound == "2378TCDD" & eu$Matrix == "Muscle" & result(eu) != 0 , ] # Zeros cannot be used in ratio estimates euRatio$Compound <- NULL euRatio <- log10(eu / euRatio)@output euRatio <- euRatio[!euRatio$Compound %in% c("2378TCDD", "2378-TCDD", "TCDD") , ] return(euRatio) } ) # Analysis: a few rows disappear here, as shown by numbers per fish species. Why? # aggregate(eut@output, by = eut@output["Fish"], FUN = length)[[2]] # [1] 1181 141 131 55 158 51 96 30 36 40 102 49 61 180 eu # [1] 1173 141 131 55 158 51 96 27 36 40 102 49 53 180 eu/eut # There are samples where TCDD concenctration is zero. #eu@data[eu@data$POP == "2378TCDD" & eu@data$euResult == 0 , c(1,4)][[1]] #eu@data[eu@data[[1]] %in% c("09K0585", "09K0698", "09K0740", "09K0748") , c(1,3,4,18)] # Some rows disappear because there is no TCDD measurement to compare with: #8 Baltic herrings, 8 sprats, 3 rainbow trouts. # Conclusion: this is ok. Total 2292 rows. ################## Data for the main congeners and species only #> unique(eu$Congener) #[1] 2378TCDD 12378PeCDD 123478HCDD 123678HCDD 123789HCDD 1234678HpCDD #[7] OCDD 2378TCDF 12378PeCDF 23478PeCDF 123478HCDF 123678HCDF #[13] 123789HCDF 234678HCDF 1234678HpCDF 1234789HpCDF OCDF CoPCB77 ... # Remove the four PCDDFs with too little data (>70% BDL) and all non-PCDDF # aggregate(eu@data$euResult, by = eu@data["POP"], FUN = function(x) mean(x == 0)) #[1] Baltic herring Sprat Salmon Sea trout Vendace #[6] Roach Perch Pike Pike-perch Burbot #[11] Whitefish Flounder Bream River lamprey Cod #[16] Trout Rainbow trout Arctic char Small herring Large herring indices <- list( Compound.TEQ2 = c("PCDDF", "PCB"), Compound.PCDDF14 = as.character(unique(euRaw$POP)[c(1:12, 14, 15)]), # 7 OCDD should be removed Fish.Fish16 = as.character(unique(euRaw$Fish_species)[c(1:4, 6:14, 17, 19, 20)]) ) #> indices #$Compound.TEQ2 #[1] "PCDDF" "PCB" # #$Compound.PCDDF14 # [1] "2378TCDD" "12378PeCDD" "123478HCDD" "123678HCDD" "123789HCDD" "1234678HpCDD" # [7] "OCDD" "2378TCDF" "12378PeCDF" "23478PeCDF" "123478HCDF" "123678HCDF" #[13] "234678HCDF" "1234678HpCDF" # #$Fish.Fish16 # [1] "Baltic herring" "Sprat" "Salmon" "Sea trout" "Roach" # [6] "Perch" "Pike" "Pike-perch" "Burbot" "Whitefish" #[11] "Flounder" "Bream" "River lamprey" "Rainbow trout" "Small herring" #[16] "Large herring" euRaw$Length<-as.numeric(as.character(euRaw$Length_mean_mm)) euRaw$Weight<-as.numeric(as.character(euRaw$Weight_mean_g)) euRaw$Large <- euRaw$Length >= 170 ggplot(euRaw@output, aes(x=Length, y=Weight))+geom_point()+ facet_wrap(~Fish_species, scales="free") #oprint(summary( # euRaw[euRaw$Fish_species %in% c("Baltic herring","Salmon") & euRaw$POP == "2378TCDD" , ], # marginals=c("Catch_location","Large","Fish_species"), # fun = "length" #)) objects.store(euRaw, eu, euRatio, indices) cat("Ovariables euRaw, eu, and euRatio and list indices stored.\n")

Bayes model for dioxin concentrations

• Model run 28.2.2017 [9]
• Model run 28.2.2017 with corrected survey model [10]
• Model run 28.2.2017 with Mu estimates [11]
• Model run 1.3.2017 [12]
• Model run 23.4.2017 [13] produces list conc.param and ovariable concentration
• Model run 24.4.2017 [14]
• Model run 19.5.2017 without ovariable concentration [15] ⇤--#: . The model does not mix well, so the results should not be used for final results. --Jouni (talk) 19:37, 19 May 2017 (UTC) (type: truth; paradigms: science: attack)

----#: . Maybe we should just estimate TEQs until the problem is fixed. --Jouni (talk) 19:37, 19 May 2017 (UTC) (type: truth; paradigms: science: comment)

• Model run 22.5.2017 with TEQdx and TEQpcb as the only Compounds [16]
• Model run 23.5.2017 debugged [17] [18] [19]
• Model run 24.5.2017 TEQdx, TECpcb -> PCDDF, PCB [20]
• Model run 11.10.2017 with small and large herring [21]

+ Show code- Hide code # This is code Op_en3104/bayes on page [[EU-kalat]] library(OpasnetUtils) library(reshape2) library(rjags) # JAGS library(ggplot2) library(MASS) # mvrnorm library(car) # scatterplotMatrix objects.latest("Op_en3104", code_name = "preprocess") # [[EU-kalat]] eu, euRatio, indices eu2 <- eu <- EvalOutput(eu) conl <- indices$Compound.TEQ2 fisl <- indices$Fish.Fish16 C <- length(conl) Fi <- length(fisl) N <- 1000 conl fisl replaces <- list( c("Chlorinated dibenzo-p-dioxins", "PCDDF"), c("Chlorinated dibenzofurans", "PCDDF"), c("Mono-ortho-substituted PCBs", "PCB"), c("Non-ortho-substituted PCBs", "PCB") ) for(i in 1:length(replaces)) { levels(eu2$Compound)[levels(eu2$Compound)==replaces[[i]][1]] <- replaces[[i]][2] } eu2 <- unkeep(eu2, prevresults = TRUE, sources = TRUE) eu2 <- oapply(eu2, cols = "TEFversion", FUN = "sum") # This goes wrong if > 1 TEFversion # Hierarchical Bayes model. # PCDD/F concentrations in fish. # It uses the TEQ sum of PCDD/F (PCDDF) as the total concentration # of dioxin and PCB respectively for PCB in fish. # PCDDF depends on age of fish, fish species and catchment area, # but we only have species now so other variables are omitted. # cong depends on fish species. eu3 <- eu2[eu2$Compound %in% conl & eu2$Fish %in% fisl & eu2$Matrix == "Muscle" , ] eu3 <- reshape( eu3@output, v.names = "euResult", idvar = "THLcode", timevar = "Compound", drop = c("Matrix"), direction = "wide" ) oprint(head(eu3)) #> colnames(eu3) #[1] "THLcode" "Fish" "euResult.PCDDF" "euResult.PCB" # Find the level of quantification for dinterval function LOQ <- unlist(lapply(eu3[3:ncol(eu3)], FUN = function(x) min(x[x!=0]))) names(LOQ) <- conl cong <- data.matrix(eu3[3:ncol(eu3)]) cong <- sapply( 1:length(LOQ), FUN = function(x) ifelse(cong[,x]==0, 0.5*LOQ[x], cong[,x]) ) mod <- textConnection(" model{ for(i in 1:S) { # s = fish sample # below.LOQ[i,j] ~ dinterval(-cong[i,j], -LOQ[j]) cong[i,1:C] ~ dmnorm(mu[fis[i],], Omega[fis[i],,]) } for(i in 1:Fi) { # Fi = fish species for(j in 1:C) { mu[i,j] ~ dnorm(mu1[j], tau1[j]) } Omega[i,1:C,1:C] ~ dwish(Omega0[1:C,1:C],S) pred[i,1:C] ~ dmnorm(mu[i,], Omega[i,,]) # Model prediction } for(i in 1:C) { # C = Compound mu1[i] ~ dnorm(0, 0.0001) tau1[i] ~ dunif(0,10000) pred1[i] ~ dnorm(mu1[i], tau1[i]) } Omega1[1:C,1:C] ~ dwish(Omega0[1:C,1:C],S) } ") jags <- jags.model( mod, data = list( S = nrow(eu3), C = C, Fi = Fi, cong = log(cong), fis = match(eu3$Fish, fisl), Omega0 = diag(C)/100000 ), n.chains = 4, n.adapt = 100 ) update(jags, 100) samps.j <- jags.samples( jags, c( 'mu', # mean by fish and compound 'Omega', # precision matrix by fish and compound 'pred', # predicted concentration by fish and compound # 'mu1', # mean prior for mu by compound 'Omega1', # precision matrix by compound # 'tau1', # precision for prior of all mu 'pred1' # predicted concentration by compound ), N ) dimnames(samps.j$mu) <- list(Fish = fisl, Compound = conl, Iter = 1:N, Chain = 1:4) #dimnames(samps.j$mu1) <- list(Compound = conl, Iter = 1:N, Chain = 1:4) dimnames(samps.j$pred) <- list(Fish = fisl, Compound = conl, Iter = 1:N, Chain = 1:4) #dimnames(samps.j$tau1) <- list(Compound = conl, Iter = 1:N, Chain = 1:4) dimnames(samps.j$pred1) <- list(Compound = conl, Iter = 1:N, Chain = 1:4) dimnames(samps.j$Omega) <- list(Fish = fisl, Compound = conl, Compound2 = conl, Iter=1:N, Chain=1:4) dimnames(samps.j$Omega1) <- list(Compound = conl, Compound2 = conl, Iter=1:N, Chain=1:4) ##### conc.param contains expected values of the distribution parameters from the model conc.param <- list( mu = apply(samps.j$mu[,,,1], MARGIN = 1:2, FUN = mean), Omega = apply(samps.j$Omega[,,,,1], MARGIN = 1:3, FUN = mean), pred.mean = apply(samps.j$pred[,,,1], MARGIN = 1:2, FUN = mean), pred.sd = apply(samps.j$pred[,,,1], MARGIN = 1:2, FUN = sd), # mu1 = apply(samps.j$mu1[,,1], MARGIN = 1, FUN = mean), # tau1 = apply(samps.j$tau1[,,1], MARGIN = 1, FUN = mean), pred1.mean = apply(samps.j$pred1[,,1], MARGIN = 1, FUN = mean), pred1.sd = apply(samps.j$pred1[,,1], MARGIN = 1, FUN = sd) ) objects.store(conc.param, samps.j) cat("Lists conc.params and samps.j stored.\n") ######################3 cat("Descriptive statistics:\n") # Leave only the main fish species and congeners and remove others conl <- indices$Compound.PCDDF14 eu <- eu[eu$Compound %in% conl & eu$Fish %in% fisl , ] oprint(summary( eu, marginals = c("Fish", "Compound"), # Matrix is always 'Muscle' function_names = c("mean", "sd") )) euRatio <- EvalOutput(euRatio) oprint(summary( euRatio, marginals = c("Fish", "Compound"), # Matrix is always 'Muscle' function_names = c("mean", "sd") )) ggplot(eu@output, aes(x = euResult, colour=Compound))+geom_density()+ facet_wrap( ~ Fish, scales = "free_y")+scale_x_log10() #stat_ellipse() ggplot(euRatio@output, aes(x = euRatioResult, colour = Compound))+geom_density()+ facet_wrap(~ Fish, scales = "free_y") ggplot(melt(exp(samps.j$pred[,,,1])), aes(x=value, colour=Compound))+geom_density()+ facet_wrap( ~ Fish,scales = "free_y")+scale_x_log10() ggplot(melt(exp(samps.j$pred1[,,1])), aes(x=value, colour=Compound))+geom_density()+ scale_x_log10() scatterplotMatrix(t(samps.j$pred[1,,,1]), main = "Predictions for all compounds for Baltic herring") ## scatterplotMatrix(t(samps.j$mu1[,,1]), main = "Means for all compounds of the generic fish") scatterplotMatrix(t(samps.j$pred1[,,1]), main = "Prediction for all compounds of the generic fish") scatterplotMatrix(t(samps.j$pred[,1,,1]), main = "Predictions for all fish species for PCDDF") scatterplotMatrix(t(samps.j$Omega[6,2,,,1]), main = "Predictions of Omega for pike and PCB") coda.j <- coda.samples( jags, c('mu', 'pred', 'Omega', 'pred1'), N ) plot(coda.j)

Initiate conc_pcddf

• Model run 19.5.2017 [22]
• Model run 23.5.2017 with bugs fixed [23]
• Model run 12.10.2017: TEQ calculation added [24]

+ Show code- Hide code # This is code Op_en3104/initiate on page [[EU-kalat]] library(OpasnetUtils) conc_pcddf <- Ovariable( "conc_pcddf", dependencies = data.frame(Name = "conc.param", Ident = "Op_en3104/bayes"), formula = function(...) { require(MASS) require(reshape2) jsp <- lapply(1:length(conc.param$mu[, 1]), FUN = function(x) { temp <- exp(mvrnorm( openv$N, conc.param$mu[x, ], solve(conc.param$Omega[x, , ]) )) dimnames(temp) <- c(list(Iter = 1:openv$N), dimnames(conc.param$mu)[2]) return(temp) }) names(jsp) <- dimnames(conc.param$mu)[[1]] jsp <- melt(jsp, value.name = "Result") colnames(jsp)[colnames(jsp) == "L1"] <- "Fish" jsp <- Ovariable( output = jsp, marginal = colnames(jsp) != "Result" ) d <- oapply(jsp, cols = "Compound", FUN = sum) d$Compound <- "TEQ" return(combine(jsp,d)) } ) objects.store(conc_pcddf) cat("Ovariable conc_pcddf stored.\n")

⇤--#: . These codes should be coherent with POPs in Baltic herring. --Jouni (talk) 12:14, 7 June 2017 (UTC) (type: truth; paradigms: science: attack)